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KMID : 0359320030430010103
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Korean Journal of Veterinary Research
2003 Volume.43 No. 1 p.103 ~ p.112
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Development of multiplex polymerase chain reaction for the detection of vancomycin resistant genotypes and Enterococcus Sp.-specific genes
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조윤상/Cho, Yun-Sang
이희수/김종만/안종삼/류판동/박용호/유한상/Lee, Hee-Soo/Kim, Jong-Man/Ahn, Jong-Sam/Ryu, Pan-Dong/Park, Yong-Ho/Yoo, Han-Sang
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Abstract
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A multiplex PCR assay, which allows simultaneous detection of vancomycin resistant genotypes and Enterococcus species-specific genes, was developed. Bancomycin resitant enterococci (VRE) from chicken and human could be detected for vanA, vanB, vanC-1, vanC-2, ddl_Efaecium and ddl_Efaecium by multiplex PCR. Eight isolates of VRE from humans (n=11) had ddl_Efaecium and vanA and 3 isolates fo the VRE had ddl_Efaecium and vanB. One isolate of VRE from chckens (n=6) had ddl_Efaecium and vanA, and 5 isolates of the VRE had only vanA. E. faecium, E. faecalis, E. gallinarum and E. casseliflavus were also confirmed for the species-specific gene by multiplex PCR. This multiplex PCR could detect E. faecuym, E. facelis, E. gallinarum, E. casseliflavus, vanA, vanB, vanC-1, and vanC-2, simultaneously. The PCR assay established in the present study can be an alternative to time-consuming biochemical tests and antibiotic suscepttibility tests of Enterococcus spp.
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